Accuracy of the ELCAD results for vaccine samples is determined to be within 2 to 9% at various levels of mercury. Apparently, Shekhar studied the effects of the concentration of low molecular weight organic compounds, i.e., ethanol (CH3CH2OH), formic acid (HCOOH) and acetic acid (CH3COOH), added to a solution of the liquid cathode on the response of the Hg I 253.7 nm emission line.15 The researcher established that the presence of 5.0 (m/v) CH3COOH in. In the absence of any CRM of the vaccine sample, the method has been validated by analyzing the same samples by Cold vapour-AAS and Electrothermal-AAS. These results show an accuracy of between 0.6 to 2.1% with an analytical precision expressed as relative standard deviation (RSD%) of between 3 to 7% based on the multiple measurements on multiple sample loadings. Biological certified reference materials, Tuna fish-IAEA-350 and Tuna fish-BCR-463 are examined for the applicability of ELCAD-AES to quantify the mercury. Analytical performance of the ELCAD-AES for the inorganic as well as the organic mercury ( methyl and thiomersal mercury) in the aqueous solutions is investigated. This method obviates the need of any sample digestion, except simple dilution followed by analysis of the vaccine samples.

A simple, rapid and sensitive method has been developed for the determination of mercury in Hepatitis-B vaccine samples using electrolyte cathode glow discharge atomic emission spectrometry (ELCAD-AES).

Shekhar elcad mercury